Transduction of human hepatocellular carcinoma cells with human &gamma;-interferon gene via retroviral vector

doi:10.3748/wjg.v4.i3.210

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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Original Article

World J Gastroenterol. Jun 15, 1998; 4(3): 210-213
Published online Jun 15, 1998. doi: 10.3748/wjg.v4.i3.210

Table 1 Secretion of IFN-γ and expression of HLA antigens by transduced hepatocellular carcinoma cells in culture

Cell lines	IFN-γ Production	Control^*	*HLA class I ^{ }*	HLA class II^△
Cell lines	(U/5 × 10⁵ cells/24 h)	MCN (% positive)^△△	MCN (% positive)	MCN (% positive)
QGY7701	0	18 (6)	159 (99)	26 (11)
QGY7701.3A	75	21 (5)	306 (100)	28 (77)
SMMC7721	0	9 (2)	200 (100)	13 (12)
SMMC7721.2C	150	21 (3)	348 (100)	14 (58)
BEL7704	0	20 (7)	34 (93)	21 (8)
BEL7404.6A	75	22 (5)	117 (100)	29 (47)
HHCL	0	10 (3)	232 (100)	12 (10)
HHCL.2C	100	22 (6)	340 (100)	37 (56)
HHCL.2D	25	14 (6)	440 (100)	12 (68)
HHCL.5C	100	11 (4)	401 (100)	19 (83)
HHCL.6C	75	15 (3)	550 (100)	10 (44)
HHCL.5D	200	10 (3)	341 (100)	10 (40)

^* Cells were stained with fluorescence isothiocyanatic-conjugated goat anti-mouse IgG (FITC-IgG) as a control. ^{* *} Cells were stained with anti-HLA class I McAb following with FITC-IgG. ^△Cells were stained with anti-HLA class II McAb following with FITC-IgG. ^△△Mean fluorescence channel number (MCN) and percentages of positive cells (in parenthese).

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Citation: Qian SB, Chen SS. Transduction of human hepatocellular carcinoma cells with human γ-interferon gene via retroviral vector. World J Gastroenterol 1998; 4(3): 210-213
URL: https://www.wjgnet.com/1007-9327/full/v4/i3/210.htm
DOI: https://dx.doi.org/10.3748/wjg.v4.i3.210

Qian SB, Chen SS. Transduction of human hepatocellular carcinoma cells with human γ-interferon gene via retroviral vector. World J Gastroenterol 1998; 4(3): 210-213 [PMID: 11819277 DOI: 10.3748/wjg.v4.i3.210]

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