Melatonin attenuates splanchnic hyperdynamic circulation in cirrhotic portal hypertension by disrupting POMK-VEGFR2 interaction

Figure 2 Melatonin alleviated bile duct ligation-induced mesenteric vascular remodeling and angiogenesis. A: Representative hematoxylin and eosin staining images of mesenteric artery. The yellow arrows represent the thickness of vascular wall; B: Quantitative analysis of the wall thickness; C: Enzyme-linked immunosorbent assay of inflammatory cytokines in mesenteric artery tissues; D: Representative immunohistochemical images of CD68 in the mesentery; E: Quantitative analysis of CD68-positive cells; F: Representative immunohistochemical images of CD31 in the mesentery; G: Quantitative analysis of CD31 staining intensity; H: Western blotting of mesenteric VEGFR-2 and its phosphorylated form; I: Quantitative analysis of VEGFR-2 phosphorylation. ^aP < 0.05. ^bP < 0.01. BDL: Bile duct ligation; MLT: Melatonin; HE: Hematoxylin and eosin; IL: Interleukin; TNF-α: Tumor necrosis factor alpha.