Identification of UPB1 as a tumor suppressor associated with immune infiltration in cholangiocarcinoma

Figure 6 UPB1 participates in tumor immune infiltration of cholangiocarcinoma. A: Correlations between UPB1 expression and tumor-infiltrating lymphocytes (TILs) in TISIDB web server; B: Top 3 TILs with greatest positive Spearman’s correlation with UPB1; C and D: Correlations between UPB1 expression and gene markers of TILs and other immune checkpoints; E: Example diagram for co-culture of HUCCT cells and T cells; F: Western blot analysis on the expressions of T cell activation markers, CD25, CD44 and CD69; G: Elisa assay on the levels of interleukin-2, interferon-γ, tumor necrosis factor-α and CXCL10. CD: Cluster of differentiation; Th: T helper; Treg: Regulatory T cells; NK: Natural killer; MDSC: Myeloid-derived suppressor cells; NKT: Natural killer T cells; DC: Dendritic cells; pDC: Plasmacytoid dendritic cells; iDC: Immature dendritic cells; CECS: Conventional endometrial carcinosarcoma; CHOL: Cholangiocarcinoma; COAD: Colon adenocarcinoma; ESCA: Esophageal carcinoma; GBM: Glioblastoma multiforme; HNSC: Head and neck squamous cell carcinoma; KICH: Kidney chromophobe; KIRC: Kidney renal clear cell carcinoma; KIRP: Kidney renal papillary cell carcinoma; LGG: Lower grade glioma; LIHC: Liver hepatocellular carcinoma; LUAD: Lung adenocarcinoma; MESO: Mesothelioma; OV: Ovarian serous cystadenocarcinoma; PAAD: Pancreatic adenocarcinoma; PCPG: Pheochromocytoma and paraganglioma; PRAD: Prostate adenocarcinoma; READ: Rectum adenocarcinoma; SARC: Sarcoma; SKCM: Skin cutaneous melanoma; STAD: Stomach adenocarcinoma; TGCT: Testicular germ cell tumor; THCA: Thyroid papillary carcinoma; UCEC: Uterine corpus endometrial carcinoma; UCS: Uterine carcinosarcoma; UVM: Uveal melanoma; OV: Over-expression; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; IL: Interleukin; IFN: Interferon; TNF: Tumor necrosis factor.