Identification of region-specific signaling modules governing Crohn’s pathogenesis via mass spectrometry-based integrative acetyl-proteomics

Figure 3 Motif analysis and abundance patterns of acetylated sites. A: Sequence logos showing conserved amino acid motifs surrounding lysine acetylation (Kac) sites (± 10 residues) in proximal-colon, mid-colon, and distal-colon tissues; B: Enrichment scores of overrepresented motifs identified in each colonic segment; C: Heatmap showing normalized abundance of quantified acetylated sites across 24 tissue samples. Each row represents an acetylated protein, and each column represents an individual sample. Color scale indicates z-score-normalized abundance (red: High, blue: Low). P-colon: Proximal-colon; M-colon: Mid-colon; D-colon: Distal-colon; CD: Crohn’s disease; JAK: Janus Kinase; STAT: Signal transducer and activator of transcription; Y: Tyrosine; W: Tryptophan; V: Valine; T: Threonine; S: Serine; R: Arginine; Q: Glutamine; P: Proline; N: Asparagine; M: Methionine; L: Leucine; K: Lysine; I: Isoleucine; H: Histidine; G: Glycine; F: Phenylalanine; E: Glutamic acid; D: Aspartic acid; C: Cysteine; A: Alanine.