Evaluation of a 3,5-diethoxycarbonyl-1,4-dihydrocollidine diet-induced mouse model in a comparative experimental study of portal hypertension

Figure 5 The 3,5-diethoxycarbonyl-1,4-dihydrocollidine-induced intrahepatic inflammatory responses contribute to elevated portal pressure. A: Immunohistochemistry staining for F4/80 in mouse livers; B: Statistical analysis of F4/80-positive staining areas; C and D: Detection of hepatic inflammatory index messenger RNA and liver enzymes in normal chow diet and 3,5-diethoxycarbonyl-1,4-dihydrocollidine groups; E and F: Detection of hepatic inflammatory index messenger RNA and liver enzymes in sham and bile duct ligation groups; G and H: Detection of hepatic inflammatory index messenger RNA and liver enzymes in oil, 8-week carbon tetrachloride (CCl₄), and 12-week CCl₄ mouse models. Sample sizes (n): 8:8 (normal chow diet vs 3,5-diethoxycarbonyl-1,4-dihydrocollidine), 8:7 (sham vs bile duct ligation), and 8:7:8 (oil vs 8-week carbon tetrachloride vs 12-week carbon tetrachloride). ^bP < 0.01. ^cP < 0.001. NS: No significant; NCD: Normal chow diet; DDC: 3,5-diethoxycarbonyl-1,4-dihydrocollidine; BDL: Bile duct ligation; CCl₄: Carbon tetrachloride; IL: Interleukin; TNF: Tumor necrosis factor; ALT: Alanine aminotransferase; AST: Aspartate aminotransferase; T-Bil: Total bilirubin; D-Bil: Direct bilirubin; TBA: Total bile acid.