Small animal ex vivo machine perfusion of the liver: A comprehensive literature review

Figure 2 Dual-vessel closed machine perfusion circuit. Perfusate is stored in the perfusate reservoir where the targeted temperature is achieved through tubing connected to the thermos unit (e.g., water bath). The perfusate is oxygenated via an oxygenator connected to a gas tank. Oxygenated perfusate, maintained at a specific temperature, is pumped through roller pumps controlled by flow and pressure regulators. Upon passing through the pumps, the perfusate goes through a heat exchanger and bubble traps before reaching its final destination: The portal vein and hepatic artery. Exiting the liver via the infrahepatic vena cava, the perfusate returns to the perfusate reservoir through tubing. The common bile duct is cannulated, and the catheter is connected to the tube for bile collection. HA: Hepatic artery; PV: Portal vein.