Xing-Pi-Qing-Gan decoction alleviates alcoholic liver disease by down-regulating DDIT3 and restoring Nrf2/HO-1 antioxidant signaling: Multi-omics and experimental evidence

Figure 7 Network pharmacology and RNA sequencing Highlights MAPK/CHOP/Nrf2 may be important targets. A: Principal component analysis; B: Volcano plot of differentially expressed mRNAs in alcoholic liver disease mice after Xing-Pi-Qing-Gan decoction intervention; C: Gene Ontology enrichment analysis of differentially expressed mRNAs; D: Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis of the differentially expressed genes from liver RNA-seq data. The top 15 significantly enriched pathways are shown; E: Gene Set Enrichment Analysis of differentially expressed mRNAs in MAPK significant pathways; F: Heatmap shows endoplasmic reticulum-related differentially expressed genes. XPQG: Xing-Pi-Qing-Gan decoction.