Xing-Pi-Qing-Gan decoction alleviates alcoholic liver disease by down-regulating DDIT3 and restoring Nrf2/HO-1 antioxidant signaling: Multi-omics and experimental evidence

Figure 3 Xing-Pi-Qing-Gan decoction-treated serum significantly reduced the oxidative stress caused by ethanol on HepG2 cells. A: JC-1 of HepG2 (40 ×); B: Reactive oxygen species levels of HepG2 (40 ×); C-E: Superoxide dismutase, glutathione and malondialdehyde levels of HepG2 (n = 3-5). ^aP < 0.01 vs the ethanol-fed group; ^bP < 0.01 vs the control group; ^cP < 0.05 vs the ethanol-fed group. Ctr: Control; EtOH: Ethanol-fed; XPQG: Xing-Pi-Qing-Gan decoction; XPQG-L: Xing-Pi-Qing-Gan decoction-low; XPQG-M: Xing-Pi-Qing-Gan decoction-medium; XPQG-H: Xing-Pi-Qing-Gan decoction-high; SOD: Superoxide dismutase; GSH: Glutathione; MDA: Malondialdehyde.