Lactate metabolism-driven tumor heterogeneity and molecular signatures in intrahepatic cholangiocarcinoma

Figure 8 Functional role of CYC1 in intrahepatic cholangiocarcinoma cell lines. A: Quantitative polymerase chain reaction validation of CYC1 knockdown efficiency in HCCC9810 and HuCCT1 cells; B and C: Cell counting kit-8 assay showing reduced proliferation following CYC1 silencing; D and E: Colony-formation assay demonstrating decreased colony number and size after CYC1 knockdown; F and G: Wound-healing assay showing impaired migratory capacity in CYC1-deficient cells; H-J: Transwell migration and invasion assays confirming that CYC1 depletion significantly suppresses motility in both intrahepatic cholangiocarcinoma cell lines. ^aP < 0.05. ^bP < 0.01. ^cP < 0.001. mRNA: Messenger RNA; OD: Optical density; NC: Negative control; Si: Small interfering RNA targeting CYC1.