Effects and mechanism of Bifidobacterium on intestinal inflammation resulting from deoxycholic acid-induced M1 polarization of macrophages

Figure 3 Immunofluorescence staining of mice colonic tissue (inducible nitric oxide synthase + F4/80, CD206+ F4/80). A: Immunofluorescence staining of mice colonic tissue (inducible nitric oxide synthase + F4/80) (scale bar, 10 μm); B: Fluorescence expression intensity (inducible nitric oxide synthase + F4/80); C: Immunofluorescence staining of mice colon tissue (CD206 + F4/80) (scale bar, 10 μm); D: Fluorescence expression intensity (CD206 + F4/80). n = 6. ^aP < 0.05. INOS: Inducible nitric oxide synthase; H-V-D-S: High-fat diet with vancomycin, deoxycholic acid, and Bifidobacterium; H-V-D: High-fat diet with vancomycin and deoxycholic acid; H-V-S: High-fat diet with vancomycin and Bifidobacterium; H-V: High-fat diet with vancomycin; H-S: High-fat diet supplemented with Bifidobacterium; H: High-fat diet; N: General.