Effects and mechanism of Bifidobacterium on intestinal inflammation resulting from deoxycholic acid-induced M1 polarization of macrophages

Figure 2 Changes in the weight and feeding pattern in mice with hematoxylin and eosin staining and immunohistochemistry of colonic tissue. A: Changes in the weight of mice of each group over time; B: Changes in the feeding pattern of the mice of each group over time; C: Hematoxylin and eosin staining of the colonic tissue of the mice of each group (scale bar: 200 ×, 100 μm; 400 ×, 60 μm); D: Colon histological scores of the mice of each group; E: Proportion of the positive area of colon immunohistochemistry in mice (%); F: Immunohistochemistry of the mice colonic tissues (F4/80) (scale bars: 50 μm). n = 6. ^aP < 0.05. H-V-D-S: High-fat diet with vancomycin, deoxycholic acid, and Bifidobacterium; H-V-D: High-fat diet with vancomycin and deoxycholic acid; H-V-S: High-fat diet with vancomycin and Bifidobacterium; H-V: High-fat diet with vancomycin; H-S: High-fat diet supplemented with Bifidobacterium; H: High-fat diet; N: General.