Danggui-Baishao herb pair protects against dextran sulfate sodium-induced colitis by modulating the Wnt/β-catenin pathway

Figure 8 The β-catenin inhibitor abolished the protective effect of the herb pair on dextran sulfate sodium-induced colitis. A: Colon length changes in mice from each group; B: Representative images of the colon length; C: Representative images of hematoxylin and eosin staining of colon tissues; D: Histological scores of hematoxylin and eosin staining of colon tissues; E: Quantitative polymerase chain reaction analysis of mRNA expression of Occludin in colon tissues; F: Quantitative polymerase chain reaction analysis of mRNA expression of leucine-rich repeat-containing G-protein coupled receptor 5, SRY-box transcription factor 9, achaete scute-like 2, telomerase reverse transcriptase, and Mucin 2 in colon tissues; G-I: Western blotting analysis of protein expression of Occludin and E-cadherin in colon tissues. For colon length measurement: n = 6, for western blotting analysis: n = 3, for quantitative polymerase chain reaction analysis: n = 5-6. ^aP < 0.05 vs dextran sulfate sodium group, ^bP < 0.01 vs dextran sulfate sodium group, ^cP < 0.001 vs dextran sulfate sodium group. ns: No significance compared with the dextran sulfate sodium + ICG-001 group. DSS: Dextran sulfate sodium; GAPDH: Glyceraldehyde 3-phosphate dehydrogenase; Lgr5: Leucine-rich repeat-containing G-protein coupled receptor 5; Sox9: SRY-box transcription factor 9; Ascl2: Achaete scute-like 2; Tert: Telomerase reverse transcriptase; Muc2: Mucin 2.