Danggui-Baishao herb pair protects against dextran sulfate sodium-induced colitis by modulating the Wnt/β-catenin pathway

Figure 5 Herb pair promoted the Wnt/β-catenin signaling activation in colitis mice. A: The overlap analysis visualized in the Venn diagram; B: The overlap analysis visualized in the heatmap; C: Gene set enrichment analysis; D: The overlap analysis visualized in the heatmap of the Wnt signal; E: Quantitative polymerase chain reaction analysis of mRNA expression of porcupine, Wnt5b, Wnt6, Wnt9a, Wnt10a, and Wnt4 in colon tissues; F-I: Western blotting analysis of protein expression of β-catenin, p- glycogen synthase kinase-3β, glycogen synthase kinase-3β, and Cyclin D1 in colon tissues; J: Representative images of immunohistochemical (IHC) of β-catenin in colon tissues; K: Quantitative analysis of IHC results. For RNA sequencing and western blotting analysis: n = 3, for quantitative polymerase chain reaction analysis: n = 5-6, for IHC measurement: n = 3. ^aP < 0.05 vs dextran sulfate sodium group, ^bP < 0.01 vs dextran sulfate sodium group, ^cP < 0.001 vs dextran sulfate sodium group. DSS: Dextran sulfate sodium; GSK-3β: Glycogen synthase kinase-3β; Porcn: Porcupine.