Danggui-Baishao herb pair protects against dextran sulfate sodium-induced colitis by modulating the Wnt/β-catenin pathway

Figure 4 Herb pair restored abnormal epithelial proliferation in colitis mice. A and B: Western blotting analysis of protein expression of leucine-rich repeat-containing G-protein coupled receptor 5 (Lgr5) in colon tissues; C: Quantitative polymerase chain reaction analysis of mRNA expression of Lgr5, SRY-box transcription factor 9, homeodomain-only protein homeobox, achaete scute-like 2, telomerase reverse transcriptase, Mucin 2, lysozyme 1, and Chromogranin A in colon tissues; D: Representative images of immunohistochemical (IHC) of Lgr5, proliferating cell nuclear antigen, Ki67 in colon tissues; E-G: Quantitative analysis of IHC results of Lgr5, proliferating cell nuclear antigen, Ki67. For western blotting analysis: n = 3, for quantitative polymerase chain reaction analysis: n = 5-6, for IHC measurement: n = 3. ^aP < 0.05 vs dextran sulfate sodium group, ^bP < 0.01 vs dextran sulfate sodium group, ^cP < 0.001 vs dextran sulfate sodium group. Lgr5: Leucine-rich repeat-containing G-protein coupled receptor 5; Sox9: SRY-box transcription factor 9; Hopx: Homeodomain-only protein homeobox; Ascl2: Achaete scute-like 2; Tert: Telomerase reverse transcriptase; Muc2: Mucin 2; Lyz1: Lysozyme 1; Chga: Chromogranin A; DSS: Dextran sulfate sodium; PCNA: Proliferating cell nuclear antigen.