Integrating proteomics, metabolomics, and molecular docking to elucidate the mechanism of Jiangtang Qingre formula against metabolic dysfunction-associated steatohepatitis

Figure 10  Validation of Lmod3 knockdown by small interfering RNA in AML12 cells (n = 3). A: Lmod3 protein expression level; B: Intracellular triglycerides content; C: Malondialdehyde level; D: Α-smooth muscle actin protein expression level; E: Cell viability. ^aP < 0.01 vs AML12 group; ^bP < 0.01 vs AML12 + free fatty acids + negative control small interfering RNA (siRNA) group; ^cP < 0.01 vs AML12 + free fatty acids + siRNA targeting mouse Lmod3 + control group. MDA: Malondialdehyde; TG: Triglycerides; si-NC: Negative control small interfering RNA; si-Lmod3: Small interfering RNA targeting mouse Lmod3; Con: Control; JQF: Jiangtang Qingre formula; FFA: Free fatty acids; α-SMA: Α-smooth muscle actin.