Ya-Han-Lu-Duan decoction reshapes tumor microenvironment to treat colitis-associated colorectal cancer by regulating arachidonic acid metabolism

Figure 4 Effects of Ya-Han-Lu-Duan on secretion and activation of myeloid-derived suppressor cells-associated and cancer-associated fibroblasts-related factors. A: Heatmap of genes related to the cytokine-cytokine receptor interaction and extracellular matrix–receptor interaction pathways; B: Enzyme-linked immunosorbent assay detection of levels of CXCL15, CXCL2, interleukin (IL)-1β, tumor necrosis factor, SPP1, IL-11, CSF3, and IL-23 in colon tissue; C: Western blot detection of COL9A3 and BMP7 expression levels in colon tissue; D: Immunofluorescence observation of FAP and CD11b⁺Gr-1⁺ expression. n = 6 for A and D, n = 20 in control group, n = 12 in colitis-associated colorectal cancer group, n = 18 in high-dose Ya-Han-Lu-Duan group for B; n = 3 for C. CAC: Colitis-associated colorectal cancer; CON: Control; YHLD-H: High-dose Ya-Han-Lu-Duan.