Zfp36 zinc finger CCCH-type inhibits gastric cancer progression by upregulating lipase G and binding its 3’ untranslated region

Figure 3 ZFP36 regulates alternative splicing of genes in AGS cells. A: Bar plot illustrating alternative splicing events governed by ZFP36; B: Scatter plot displaying top enriched Gene Ontology biological processes among genes that undergo alternative spliced; C-E: ZFP36-mediated alternative splicing events for WWOX (A3SS), METTL2B (ES), and NEDD9 (A3SS) are presented. Left panel: Integrative genomics viewer-sashimi plot illustrating binding sites and regulated splicing events along the mRNA transcript. Upper panel displays read coverage for each regulated alternative splicing event; gene transcript structures are depicted beneath. Right panel: Structural diagrams of alternative splicing events. Bottom portion of the right panel shows confirmation via RNA sequencing and reverse transcription-quantitative polymerase chain reaction. ^aP < 0.05 vs negative control group, ^bP < 0.01 vs negative control group, ^dP < 0.0001 vs negative control group. NC: Negative control; OE: Overexpression; GO: Gene Ontology.