Zfp36 zinc finger CCCH-type inhibits gastric cancer progression by upregulating lipase G and binding its 3’ untranslated region

Figure 2 ZFP36 regulates gene expression in AGS cells. A: Principal component analysis based on fragments per kilobase of transcript per million mapped reads values of all detected genes following ZFP36 overexpression. Ellipses for each group represent the confidence ellipse; B: Volcano plot showing all differentially expressed gene (DEGs) between the overexpression-ZFP36 and NC samples; C: Hierarchical clustering heatmap showing expression levels of all DEGs; D: Scatter plot exhibiting the most enriched Gene Ontology biological process results for up-regulated DEGs; E: Scatter plot exhibiting the most enriched Gene Ontology biological process results for down-regulated DEGs; F: Bar plot showing the expression pattern and statistical differences of DEGs, as determined by RNA sequencing and reverse transcription-quantitative polymerase chain reaction validation. ^cP < 0.001 vs negative control group, ^dP < 0.0001 vs negative control group. PCA: Principal component analysis; NC: Negative control; OE: Overexpression; GO: Gene Ontology; FPKM: Fragments per kilobase of transcript per million mapped reads.