Gluten-free diet reduces severity of dextran sulfate sodium-induced colitis in BALB/c mice

Figure 6 Effect of gluten-free diet on forkhead box P3⁺ regulatory T cells and interleukin-10⁺ T helper cells in mesenteric lymph nodes and spleen of dextran sulfate sodium-treated mice. A: Proportion of cluster of differentiation (CD) 4⁺ forkhead box P3 (Foxp3)⁺ cells within T cells (CD3⁺-gated); B: Example dot plots of CD4⁺ Foxp3⁺ regulatory T cells in mesenteric lymph nodes (MLN); C: Proportion of interleukin (IL)-10⁺ T helper cells (CD3⁺ CD4⁺-gated) in MLN and spleen of female BALB/c mice fed gluten-free diet and standard diet (n = 8) that were treated for 7 days with 2.5% dextran sulfate sodium; D: IL-10⁺ (CD3⁺ CD4⁺) T helper cells. Data are expressed as mean ± SD. ^aP < 0.05. ^bP < 0.01. P values represent an example of two independent experiments. MLN: Mesenteric lymph nodes; STD: Standard diet; GFD: Gluten-free diet; CD: Cluster of differentiation; TCR: T cell receptor; IL: Interleukin; Foxp3: Forkhead box P3; SSC-A: Side scatter-area; Treg: Regulatory T cells; PE-Cy7: Phycoerythrin-cyanine 7; Th: T helper cell.