Dendrobium officinale polysaccharides attenuates spasmolytic polypeptide-expressing metaplasia via dual modulation of gastric epithelial cell fate and macrophage polarization

Figure 6 The effect of Dendrobium officinale polysaccharides on IL-13/STAT6 signal pathway on bone marrow-derived macrophages cell. A: Flow cytometry analysis of M2 macrophage polarization; B: Flow cytometry result analysis by flowjo software; C: Genes expression of macrophage marker and interleukin (IL)-13, IL-13Ra1, IL-13Ra2, ST2 by real-time polymerase chain reaction; D: Protein expression of macrophage marker; E: Relative protein quantification (macrophage marker) performed using Image Lab 6.0; F: Protein expression of IL-13, STAT6 and p-STAT6; G: Relative protein quantification (IL-13, STAT6 and p-STAT6) performed using Image Lab 6.0. The data are represented as mean ± SD, n = 3. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001, vs control; ^dP < 0.05, ^eP < 0.01, ^fP < 0.001, vs spasmolytic polypeptide-expressing metaplasia model. H-DOP: High-dose Dendrobium officinale polysaccharides; L-DOP: Low-dose Dendrobium officinale polysaccharides; M-DOP: Middle-dose Dendrobium officinale polysaccharides; IL: Interleukin.