Dendrobium officinale polysaccharides attenuates spasmolytic polypeptide-expressing metaplasia via dual modulation of gastric epithelial cell fate and macrophage polarization

Figure 3 The co-expression of aquaporin 5 and Ki67, gastric intrinsic factor and Ki67 in mouse gastric mucosal cells. A: Immunofluorescence (IF) analysis the co-expression of aquaporin 5 (AQP5) and Ki67; B: Statistical analysis of IF results (Ki67+ AQP5+) by using ImageJ software; C: Real-time polymerase chain reaction analysis of AQP5; D: IF analysis of the co-expression of gastric intrinsic factor and Ki67; E: Statistical analysis of IF results (gastric intrinsic factor + AQP5+) by using ImageJ software. ^aP < 0.05, ^cP < 0.001, vs control; ^dP < 0.05, ^fP < 0.001, vs spasmolytic polypeptide-expressing metaplasia model. Ctrl: Control; SPEM: Spasmolytic polypeptide-expressing metaplasia; WFC: Weifuchun capsule; H-DOP: High-dose Dendrobium officinale polysaccharides; L-DOP: Low-dose Dendrobium officinale polysaccharides; AQP5: Aquaporin 5; GIF: Gastric intrinsic factor.