Dendrobium officinale polysaccharides attenuates spasmolytic polypeptide-expressing metaplasia via dual modulation of gastric epithelial cell fate and macrophage polarization

Figure 2 Analysis of parietal, chief cell and spasmolytic polypeptide-expressing metaplasia markers. A: Immunofluorescence (IF) analysis of the parietal cell marker ATP4α; B: IF analysis of the chief cell marker Mist1; C: Statistical analysis of IF results (ATP4α) by using ImageJ software; D: Statistical analysis of IF results (Mist1) by using ImageJ software; E and F: IF analysis of spasmolytic polypeptide-expressing metaplasia markers trefoil factor 2 and GS II; G and H: Statistical analysis of IF results (TFF2 and GS II) by using ImageJ software; I and J: Real-time polymerase chain reaction analysis of ATP4α, Mist1, TFF2 and Gpx2. The data are represented as mean ± SD, IF analysis, n = 3. Real-time polymerase chain reaction analysis, n = 6. ^aP < 0.05, ^cP < 0.001, vs control; ^dP < 0.05, ^eP < 0.01, ^fP < 0.001, vs spasmolytic polypeptide-expressing metaplasia model. Ctrl: Control; SPEM: Spasmolytic polypeptide-expressing metaplasia; WFC: Weifuchun capsule; H-DOP: High-dose Dendrobium officinale polysaccharides; L-DOP: Low-dose Dendrobium officinale polysaccharides.