Microbiota-nutrition sequential therapy for refractory radiation proctitis: A case report

Figure 5 Temporal shifts in MetaCyc pathway abundances of the patient’s gut microbiome pre- and post-faecal microbiota transplantation. A: Z-score normalized heatmap showing MetaCyc pathway abundance dynamics at pre-fecal microbiota transplantation (FMT), FMT-1 and FMT-2 (orange = upregulation, blue = downregulation); B: Aggregated relative abundances of five core functional categories: Branched-chain amino acid synthesis, short-chain fatty acid synthesis, carbohydrate metabolism, lipid metabolism, and toxic degradation. Functional profiles were predicted via PICRUSt2 from serial 16S rRNA sequencing data; all analyses are descriptive owing to the single-case design. AA: Amino acid; SCFA: Short-chain fatty acid; pre-FMT: Baseline before fecal microbiota transplantation; FMT-1: 1 month after the first fecal microbiota transplantation; FMT-2: 2 months after the second fecal microbiota transplantation.