FSCN1/ACK1 axis-dependent M2 macrophage polarization fuels colorectal cancer progression

Figure 7 Fascin actin-bundling protein-1 silencing inhibits colon cancer growth and reshapes the tumor immune microenvironment in vivo. A: Representative tumor photos; B: Tumor growth curve; C: End point tumor weight; D: Immunohistochemistry staining of fascin actin-bundling protein-1, activated Cdc42-associated kinase 1, and Ki67. Scale bar = 200 μm; E: Flow cytometry analysis of polarization status of macrophages in tumors; F and G: Immunofluorescence staining images of Inducible nitric oxide synthase and arginase 1, with DAPI counterstaining of the cell nucleus. Scale bar = 50 μm; H and I: The mRNA levels of interleukin-1β and interleukin-10. ^bP < 0.01. ^cP < 0.001. FSCN1: Fascin actin-bundling protein-1; iNOS: Inducible nitric oxide synthase; Arg1: Arginase 1; ACK1: Activated Cdc42-associated kinase 1; NC: Non-targeting control; IL: Interleukin.