FSCN1/ACK1 axis-dependent M2 macrophage polarization fuels colorectal cancer progression

Figure 5 Activated Cdc42-associated kinase 1 silencing inhibits M2 phenotype of macrophages. A: Flow cytometry analysis of the ratio of CD86⁺ (M1) and CD206⁺ (M2) in macrophages; B-E: Enzyme-linked immunosorbent assay was used to detect the secretion levels of M1 and M2 macrophage cytokines; F-H: Protein expression of inducible nitric oxide synthase and arginase 1. ^cP < 0.001. PMA induced differentiation of THP-1 macrophages was treated with conditioned medium containing SW620, SW620-si-NC, and SW620-si-CK1 cells, respectively. TNF-α: Tumor necrosis factor-α; TGF-β1: Transforming growth factor-β1; iNOS: Inducible nitric oxide synthase; Arg1: Arginase 1; ACK1: Activated Cdc42-associated kinase 1; NC: Non-targeting control; CM: Conditioned medium; IL: Interleukin.