FSCN1/ACK1 axis-dependent M2 macrophage polarization fuels colorectal cancer progression

Figure 3 Knockdown of Fascin actin-bundling protein-1 inhibits M2 phenotype of macrophages. A-C: The mRNA expression levels of M1 markers NOS2, tumor necrosis factor-α, and interleukin-1β; D-F: The mRNA expression levels of M2 markers arginase 1, CD206, and interleukin-10; G and H: Immunofluorescence staining of M1 marker CD86 and M2 marker CD163. Scale bar = 50 μm. ^cP < 0.001. THP-1 cells were induced to differentiate into macrophages by PMA and treated with conditioned medium containing SW620, SW620-si-NC, and SW620-si-FSCN1 cells, respectively. TNF-α: Tumor necrosis factor-α; FSCN1: Fascin actin-bundling protein-1; CM: Conditioned medium; Arg1: Arginase 1; IL: Interleukin; NC: Non-targeting control.