Emodin ameliorates cholestatic liver injury by regulating bile acid metabolism and gut microbiota in mice

Figure 10  Microbial communities from emodin-treated mice could ameliorate α-naphthylisothiocyanate-induced cholestatic liver injury. A: Experimental design scheme; B: Observation of liver and gallbladder tissue morphology; C: Representative images of hematoxylin and eosin staining liver sections, magnification 200 ×, n = 3 (scale bar: 50 μm); D: Serum alanine transaminase, aspartate aminotransferase and total bilirubin levels. Data were analyzed via two-tailed Student’s t-test, n = 6. ^aP < 0.01 vs control group, ^bP < 0.001 vs control group, ^cP < 0.05 vs α-naphthylisothiocyanate group, and ^dP < 0.01 vs α-naphthylisothiocyanate group. ALT: Alanine transaminase; AST: Aspartate aminotransferase; TBIL: Total bilirubin; Cont: Control; Emo: Emodin; ANIT: Α-naphthylisothiocyanate; ABX: Antibiotics.