Emodin ameliorates cholestatic liver injury by regulating bile acid metabolism and gut microbiota in mice

Figure 7 Effect of emodin on distal ileum bile acids in mice with α-naphthylisothiocyanate-induced cholestasis liver injury. A: Changes of total bile acids (BAs), primary BAs, secondary BAs, taurine-conjugated BAs (T-BAs) and unconjugated BAs (U-BAs); B: Changes of U-BAs; C: Changes of T-BAs; D: Ratio of primary BAs to secondary BAs in the ileum; E: Ratio of T-BAs to U-BAs in the ileum. Data were analyzed via two-tailed Student’s t-test, n = 8. ^aP < 0.05 vs control group, ^bP < 0.01 vs control group, ^cP < 0.05 vs α-naphthylisothiocyanate group, and ^dP < 0.01 vs α-naphthylisothiocyanate group. Cont: Control; Emo: Emodin; ANIT: Α-naphthylisothiocyanate; Σ BAs: Total bile acids; 1° BAs: Primary bile acids; 2° BAs: Secondary bile acids; T-BAs: Taurine-conjugated bile acids; U-BAs: Unconjugated bile acids; CA: Cholic acid; MCA: Muricholic acid; DCA: Deoxycholic acid; CDCA: Chenodeoxycholic acid; HCA: Hyocholic acid; HDCA: Hyodesoxycholic acid; UDCA: Ursodeoxycholic acid; MDCA: Murideoxycholic acid; TMCA: Tauro-β-muricholic acid; TCA: Taurocholic acid; TCDCA: Taurochenodeoxycholic acid; THCA: Taurohyocholic acid; TMDCA: Tauromurideoxycholic acid; TUDCA: Tauroursodeoxycholic acid; TDCA: Taurodeoxycholic acid; THDCA: Taurochenodeoxycholic acid.