EIF5B/mitogen-activated protein kinase 1 axis drives tumor progression and confers dual resistance to chemotherapy and immunotherapy in colonic adenocarcinoma

Figure 3 Eukaryotic translation initiation factor 5B drove colonic adenocarcinoma cell migratory and invasive phenotypes. A and B: Transwell migration and invasion assays of eukaryotic translation initiation factor 5B (EIF5B)-overexpressing colonic adenocarcinoma (COAD) cells; C and D: Transwell migration and invasion assays of EIF5B-knockdown COAD cells. All photomicrographs were captured at 200 × magnification. n = 3 independent experiments, with values expressed as the mean ± SD. Independent-samples t-test and non-parametric tests were used for two-group comparisons. ^aP < 0.01. ^bP < 0.001. LV-EIF5B: Lentivirus expressing EIF5B; LV-NC: Lentivirus negative control; NC: Negative control; sh-EIF5B: Short hairpin RNA targeting EIF5B; sh-NC: Short hairpin RNA negative control.