EIF5B/mitogen-activated protein kinase 1 axis drives tumor progression and confers dual resistance to chemotherapy and immunotherapy in colonic adenocarcinoma

Figure 2 Eukaryotic translation initiation factor 5B enhanced colonic adenocarcinoma cell proliferation and drove tumor growth. A: Correlation of individual genes with eukaryotic translation initiation factor 5B (EIF5B) expression was assessed using three independent datasets; B: Gene Ontology-Biological Process enrichment analysis of EIF5B was conducted using three independent datasets; C: mRNA levels of EIF5B in the colonic adenocarcinoma (COAD) cell lines; D-G: Assessment of EIF5B expression at mRNA and protein levels across transfected COAD cell lines; H and I: Proliferation of EIF5B-overexpressing COAD cells were investigated; J and K: Proliferation of EIF5B-knockdown COAD cells were investigated; L: Tumor growth curves (n = 6 per group); M: Representative tumor images in nude mice (n = 6 per group); N: Tumor weights (n = 6 per group). n = 3 independent experiments (n = 4 for Figure 2C), with values expressed as the mean ± SD. Group comparisons were conducted as follows: Independent-samples t-tests or non-parametric tests for two groups; one-way analysis of variance (ANOVA) or non-parametric tests for single-factor multi-group comparisons; and two-way ANOVA for experiments with two independent factors. HCT-116 and SW948 were transfected with short hairpin RNAs targeting EIF5B (sh-EIF5B), which were designated as sh-1, sh-2, and sh-3. ^aP < 0.05. ^bP < 0.01. ^cP < 0.001. ^dP < 0.0001. TCGA: The Cancer Genome Atlas; LV-EIF5B: Lentivirus expressing EIF5B; LV-NC: Lentivirus negative control; NC: Negative control; sh-NC: Short hairpin RNA negative control.