Study on the adhesive effect of deoxyshikonin on Helicobacter pylori

Figure 7 Exogenous urease enhances bacterial adhesion to BGC823 cells. A and B: Representative fluorescence images and quantification of Helicobacter pylori 26695 adhesion to BGC823 cells in the presence of exogenous urease; C and D: Representative fluorescence images and quantitative analysis of Helicobacter pylori 26695Δcfa adhesion to BGC823 cells in the presence of exogenous urease; E and F: Representative fluorescence images and quantitative analysis of the adhesion of the Newman strain to BGC823 cells in the presence of exogenous urease. BGC823 cells are shown in rhodamine red, bacteria are labelled with SYTO9 green, and merged images show bacterial attachment to host cells. ^aP < 0.05 vs phosphate-buffered saline, ^bP < 0.01 vs phosphate-buffered saline, ^cP < 0.001 vs phosphate-buffered saline, ^eP < 0.05 vs 0.25 μg/mL urease, ^fP < 0.01 vs 0.25 μg/mL urease, ^gP < 0.001 vs 0.25 μg/mL urease, ⁱP < 0.05 vs 1 μg/mL urease. PBS: Phosphate-buffered saline.