Study on the adhesive effect of deoxyshikonin on Helicobacter pylori

Figure 5 Deoxyshikonin reduces the expression of Helicobacter pylori adhesion- and urease-related genes. A: Reverse transcription-quantitative polymerase chain reaction analysis of the relative mRNA expression levels of SabA, BabA, ureA, and ureB after deoxyshikonin treatment; ^dP < 0.0001 vs the control group; B: Representative western blot showing UreB protein expression in Helicobacter pylori treated with 0, 4, and 16 μg/mL deoxyshikonin; C: Quantification of UreB protein expression normalised to total protein loading; ^bP < 0.01 vs the 0 μg/mL deoxyshikonin group, ^dP < 0.0001 vs the 0 μg/mL deoxyshikonin group, ^gP < 0.001 vs the 4 μg/mL deoxyshikonin; D: Surface plasmon resonance sensorgrams showing the binding interaction between deoxyshikonin and UreB protein.