Study on the adhesive effect of deoxyshikonin on Helicobacter pylori

Figure 4 Deoxyshikonin-mediated inhibition of Helicobacter pylori urease activity. A and B: Representative phenol red colorimetric images and quantitative analysis of deoxyshikonin-mediated urease inhibition in the G27 strain. ^aP < 0.05 vs phosphate-buffered saline, ^bP < 0.01 vs phosphate-buffered saline, ^cP < 0.001 vs phosphate-buffered saline, ^eP < 0.05 vs acetohydroxamic acid, and ^fP < 0.01 vs acetohydroxamic acid; C: Relative mRNA expression measured by reverse transcription-quantitative polymerase chain reaction in the standard Helicobacter pylori (H. pylori) 26695 strain and the mutant strain 26695Δcfa. ^bP < 0.01 vs control, ^dP < 0.0001 vs control; D: Inhibition-zone results for the standard H. pylori 26695 strain and the mutant strain H. pylori 26695Δcfa following exposure to different concentrations of deoxyshikonin; E: Inhibition rate of deoxyshikonin against urease activity in the G27 strain, determined using a urease assay kit. ^cP < 0.001 vs phosphate-buffered saline, ^dP < 0.0001 vs phosphate-buffered saline, and ^jP < 0.001 vs acetohydroxamic acid. PBS: Phosphate-buffered saline; AHA: Acetohydroxamic acid.