Spatiotemporal atlas of internal hemorrhoids in rats elucidated using integrated single-cell RNA sequencing and spatial transcriptomics

Figure 6 Spatial transcriptome profiling of internal hemorrhoids. A: Images of hematoxylin and eosin staining; B: Dot plots of the spatial transcriptomic results. The clustering results from Seurat are overlaid on tissue sections; each dot indicates one spot, and different colors indicate each cluster; C: Maps of cell type annotations; D: Maps of the spatial deconvolution analysis of fibroblast subsets; E: Bubble plot of the top 3 marker genes per group; F: Maps showing the results of the dimensionality reduction clustering analysis; G: Maps showing the spatial localization of highly expressed genes; darker red dots indicate genes with higher expression in the spots; H: Violin plots. Ctrl: Control group; Model-1w: 1 week of modeling; Model-2w: 2 weeks of modeling.