Spatiotemporal atlas of internal hemorrhoids in rats elucidated using integrated single-cell RNA sequencing and spatial transcriptomics

Figure 4 Plots of the results of the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses. The Y-axes indicate the names of the Gene Ontology (GO) terms or Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways; the X-axes indicate the Rich factors (higher values indicate stronger enrichment). The dot size represents the number of genes; the dot color corresponds to the range of adjust P values. Adjust P < 0.05 was considered to indicate statistical significance. A: GO enrichment analysis of upregulated genes in Igfbp5-fibroblast [1 week of modeling (Model-1w) vs control group (Ctrl)]; B: GO enrichment analysis of upregulated genes in Protomyofibroblast (Model-1w vs Ctrl); C: KEGG pathway enrichment analysis of upregulated genes in three fibroblast subsets (Igfbp5-fibroblast, protomyofibroblast, and fascia-fibroblast) (Model-1w vs Ctrl); D: KEGG pathway enrichment analysis of upregulated genes in three macrophage subsets (M2 macrophage, Cd74-expressing macrophage, and monocyte-derived macrophage) (Model-1w vs Ctrl); E: KEGG pathway enrichment analysis of upregulated genes in three SMC subsets (fibroblast-smooth muscle cell (SMC), contractile-SMC, and synthetic-SMC) (Model-1w vs Ctrl). GO: Gene Ontology; KEGG: Kyoto Encyclopedia of Genes and Genomes.