Epitranscriptomic regulation of the COX-2/PGE2 axis by hnRNPA2B1 promotes esophageal squamous cell carcinoma progression

Figure 3 Transcriptomic analyses identify prostaglandin-endoperoxide synthase 2/cyclooxygenase-2 as a downstream target of hnRNPA2B1 in esophageal carcinoma. A: Volcano plot of differentially expressed genes in Eca109 cells transduced with control short hairpin RNA or hnRNPA2B1 short hairpin RNA, based on bulk RNA-seq; B: Heatmap showing the relative expression of prostaglandin-endoperoxide synthase 2 (PTGS2) and HnRNPA2B1 in control short hairpin RNA and hnRNPA2B1 short hairpin RNA samples; C: Gene Ontology enrichment analysis of HNRNPA2B1-regulated differentially expressed genes, presented as a bubble plot; D: Pan-cancer analysis of PTGS2 expression in normal (left) and tumor (right) tissues from The Cancer Genome Atlas, with the esophageal cohort highlighted; E: Distribution of PTGS2 and HnRNPA2B1 expression in normal, primary tumor, and metastatic tissues based on The Cancer Genome Atlas data; F: Correlation analysis between HnRNPA2B1 and PTGS2 expression in esophageal carcinoma samples. Statistical significance was assessed as indicated in each panel. Pearson’s correlation analysis was used in (F). ^aP < 0.05. shCON: Control short hairpin RNA; shA2B1: HnRNPA2B1 short hairpin RNA; PTGS2: Prostaglandin-endoperoxide synthase 2.