Epitranscriptomic regulation of the COX-2/PGE2 axis by hnRNPA2B1 promotes esophageal squamous cell carcinoma progression

Figure 2 HnRNPA2B1 promotes esophageal squamous cell carcinoma cell proliferation, migration, and invasion in vitro. A: Western blotting analysis of hnRNPA2B1 knockdown efficiency in Eca109 and KYSE150 cells transduced with control short hairpin RNA or three independent hnRNPA2B1-targeting short hairpin RNAs (sh56-1, sh57-12, and sh58-1), with β-actin as the loading control; B: Relative hnRNPA2B1 mRNA expression in Eca109 and KYSE150 cells after hnRNPA2B1 knockdown with sh56-1, as determined by reverse transcription-quantitative polymerase chain reaction; C: Western blotting analysis of hnRNPA2B1 overexpression in Eca109, KYSE150, and Het-1A cells transduced with negative control or hnRNPA2B1 vector (overexpression), with β-actin as the loading control; D: Relative hnRNPA2B1 mRNA expression in Eca109, KYSE150, and Het-1A cells after hnRNPA2B1 overexpression, as determined by reverse transcription-quantitative polymerase chain reaction; E: Cell counting kit-8 assays showing the effects of hnRNPA2B1 knockdown or overexpression on the proliferation of Eca109, KYSE150, and Het-1A cells; F: Representative images of colony formation assays in Eca109 cells after hnRNPA2B1 knockdown or overexpression; G: Quantification of colony numbers in (F); H: Representative images of Transwell migration and invasion assays in Eca109 cells after hnRNPA2B1 knockdown or overexpression; I: Quantification of migrated and invaded cells in (H); J: Representative images of wound-healing assays in Eca109 cells at 0, 12, and 24 hours after hnRNPA2B1 knockdown or overexpression; K: Quantification of wound closure in (J). Data are presented as mean ± SD. Statistical significance was determined by Student’s t-test for two-group comparisons or one-way ANOVA for multiple-group comparisons, as appropriate. ^aP < 0.05, ^bP < 0.01, and ^cP < 0.001. NC: Negative control; OE: Overexpression; shCON: Control short hairpin RNA; shA2B1: HnRNPA2B1 short hairpin RNA.