ALDH2 deficiency in pancreatic acinar cells aggravates acute pancreatitis by promoting NLRP3 inflammasome activation through autophagy

Figure 8 Alda-1 reduced nod-like receptor family pyrin domain-containing 3 inflammasome components in an autophagy-dependent manner. Cells were pretreated with Alda-1 (20 μM) for 4 hours prior to caerulein stimulation (100 nM), and chloroquine (25 μM) was added during the final 2 hours of caerulein stimulation. A-D: Immunoblot analysis of nod-like receptor family pyrin domain-containing 3, apoptosis associated speck-like protein and caspase-1 for cells in each group (n = 3 per group); E-G: Immunoblot analysis of P62 and microtubule-associated protein light chain 3B for cells in each group (n = 3 per group). ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. NS: Not significant; CER: Caerulein; LC3B: Light chain 3B; CQ: Chloroquine; NLRP3: Nod-like receptor family pyrin domain-containing 3; ASC: Associated speck-like protein.