ALDH2 deficiency in pancreatic acinar cells aggravates acute pancreatitis by promoting NLRP3 inflammasome activation through autophagy

Figure 7 Aldehyde dehydrogenase 2 deficiency attenuated rapamycin-induced nod-like receptor family pyrin domain-containing 3 inflammasome inhibition in caerulein-stimulated pancreatic acinar cells. MPC-83 cells were transfected with aldehyde dehydrogenase 2 (ALDH2)-siRNA and NC-siRNA. Cells were then pre-treated with the autophagy inducer rapamycin (100 nM) for 1 hour, then stimulated with caerulein (100 nM) for 24 hours before sample collection. A-C: Supernatant cell culture medium interleukin (IL)-1β, IL-6 and IL-18 levels in each group (n = 3 per group); D-G: Immunoblot analysis of nod-like receptor family pyrin domain-containing 3 (NLRP3), apoptosis associated speck-like protein (ASC) and caspase-1 for cells in each group (n = 3 per group); H: Immunofluorescence analysis of NLRP3 (red) - ASC (green) and NLRP3 (red) - caspase-1 (green) for cells in each group. Scale bar: 20 μm, magnification, 10 μm. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. CER: Caerulein; Rapa: Rapamycin; IL: Interleukin; ALDH2: Aldehyde dehydrogenase 2; NLRP3: Nod-like receptor family pyrin domain-containing 3; ASC: Associated speck-like protein.