ALDH2 deficiency in pancreatic acinar cells aggravates acute pancreatitis by promoting NLRP3 inflammasome activation through autophagy

Figure 5 Aldehyde dehydrogenase 2 deficiency aggravated nod-like receptor family pyrin domain-containing 3 inflammasome activation in caerulein-stimulated pancreatic acinar cells. MPC-83 cells were transfected with aldehyde dehydrogenase 2 (ALDH2)-siRNA and NC-siRNA. The induction of acute pancreatitis (AP) was established by caerulein (100 nM) treatment. A: The expression of ALDH2 in ALDH2-siRNA treated cells and their corresponding controls by immunoblotting (n = 3 per group); B-D: Supernatant cell culture medium interleukin (IL)-1β, IL-6 and IL-18 Levels in each group (n = 3 per group); E-H: Immunoblot analysis of nod-like receptor family pyrin domain-containing 3 (NLRP3), apoptosis associated speck-like protein (ASC) and caspase-1 for cells in each group (n = 3 per group); I: Immunofluorescence analysis of NLRP3 (red) - ASC (green) and NLRP3 (red) - caspase-1 (green) for cells in each group. Scale bar: 20 μm, magnification, 10 μm. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. NS: Not significant; CER: Caerulein; IL: Interleukin; ALDH2: Aldehyde dehydrogenase 2; NLRP3: Nod-like receptor family pyrin domain-containing 3; ASC: Associated speck-like protein.