ARPC1B contributes to colorectal cancer progression and is associated with programmed death-ligand 1 expression

Figure 5 ARPC1B regulates programmed death-ligand 1 expression in colorectal cancer. A: The relative protein expression level of programmed death-ligand 1 (PD-L1) in HT29-negative control (NC)-ARPC1B or HT29-ARPC1B-short hairpin RNA (shRNA) (sh-ARPC1B) xenograft nude mice models; B: The relative protein expression level of PD-L1 in SW620-NC-ARPC1B or SW620-sh-ARPC1B xenograft nude mice models; C: The representative immunohistochemistry results of PD-L1 in HT29-NC-ARPC1B or HT29-sh-ARPC1B xenograft nude mice models; D: The representative immunohistochemistry results of PD-L1 in SW620-NC-ARPC1B or SW620-sh-ARPC1B xenograft nude mice models; E: The co-immunoprecipitation (Co-IP) experiments of ARPC1B and PD-L1 in HT29 cells; F: The Co-IP experiments of ARPC1B and PD-L1 in SW620 cells (n = 3). scale bar = 100 μm. ^cP < 0.001. NC: Negative control; sh-ARPC1B: ARPC1B-short hairpin RNA; PD-L1: Programmed death-ligand 1; IP: Immunoprecipitation; IB: Immunoblotting; IgG: Immunoglobulin G.