Synergistic anti-hepatoma effect of triptolide and quercetin via co-inhibition and interaction with Janus kinase and mammalian target of rapamycin signal pathway

Figure 8 Apoptotic effects of triptolide and/or quercetin in HepG2 cell line by fluorescence activated cell sorter analysis. A: Annexin V-FITC/PI was performed to measure the apoptosis and then deal with FlwoJo 10. The pictures showed the treated cells in the four quadrants; B and C: The percentage of apoptotic cells in difference phases in 24 hours or 48 hours. Percentages of negative represent viable cells, annexin V-positive represent early apoptotic cells, PI-positive represent necrotic cells, or annexin V and PI double-positive represent late apoptotic cells. The tests were performed at least three independent parallel experiments. Data was presented as means ± SE of three independent experiments. ^aP < 0.05. TP: Triptolide; QU: Quercetin.