Single-cell and bulk transcriptomics with machine learning decode B cell hub genes and diagnostic biomarkers in anal fistula

Figure 12  Flow cytometric identification and gene expression analysis of B cells. A: Gating strategy for B cell isolation in healthy controls; B: Gating strategy for B cell isolation in samples of anal fistula. Cells were identified as CD19⁺ CD3^- to exclude T cells and ensure high purity of the B cell population; C: Reverse transcription-quantitative PCR analysis of LINC-PINT, LRBA, and SYK expression levels in the sorted CD19⁺ CD3^- B cells. ^aP < 0.01. AF: Anal fistula; HC: Healthy control.