Single-cell and bulk transcriptomics with machine learning decode B cell hub genes and diagnostic biomarkers in anal fistula

Figure 10  CellChat analysis of LRBA⁺ B cells in anal fistula. A: CellChat interaction network showing the number of intercellular interactions among all cell types. LRBA⁺ B cells exhibited a greater number of interactions compared to LRBA^- B cells; B: Communication strength analysis revealing that LRBA⁺ B cells possess higher overall interaction strength with other cell types; C: Heatmaps of outgoing and incoming signaling patterns across all cell types. LRBA⁺ B cells demonstrated enhanced outgoing signaling activity, particularly in classic immune and inflammatory pathways; D: The ligand-receptor interactions between different cell types and LRBA^+/- B cells.