Single-cell and bulk transcriptomics with machine learning decode B cell hub genes and diagnostic biomarkers in anal fistula

Figure 8 Cellular localization and pseudotime dynamics of key genes in B cells. A-C: Uniform manifold approximation and projection plots showing the expression patterns of LINC-PINT, LRBA, and SYK across all cell types in anal fistula samples; D: Monocle-based pseudotime trajectory analysis of B cells, identifying a continuous developmental path divided into nine states, with color gradients representing inferred pseudotime progression; E-G: Expression trends of LINC-PINT, LRBA, and SYK along the pseudotime axis. LINC-PINT shows high expression in early pseudotime, declining thereafter, suggesting a role in early B cell activation. LRBA shows a gradual decrease in expression, indicating a role in early-to-mid B cell stages. SYK expression increases at later pseudotime stages, implying involvement in terminal differentiation or immunoregulatory functions of B cells. UMAP: Uniform manifold approximation and projection.