Single-cell and bulk transcriptomics with machine learning decode B cell hub genes and diagnostic biomarkers in anal fistula

Figure 6 Immune correlation and functional enrichment analysis of LINC-PINT, LRBA, and SYK. A: Correlation between the expression of LINC-PINT, LRBA, SYK and the abundance of various immune cells, based on single-sample gene set enrichment analysis (GSEA) scores in anal fistula samples; B: Correlation heatmap of LINC-PINT, LRBA, SYK with immune-related functions, including cytokine receptors, antigen processing, and co-stimulatory molecules; C and D: Single-gene GSEA enrichment analysis for LINC-PINT showing significant enrichment in TGF-β signaling pathway and MAPK signaling pathway; E and F: GSEA results for LRBA indicating enrichment in Primary immunodeficiency and T cell receptor signaling pathway; G and H: GSEA results for SYK showing significant enrichment in cytokine-cytokine receptor interaction and Natural killer cell mediated cytotoxicity. ^aP < 0.05; ^bP < 0.01; ^cP < 0.001. KEGG: Kyoto Encyclopedia of Genes and Genomes.