Single-cell and bulk transcriptomics with machine learning decode B cell hub genes and diagnostic biomarkers in anal fistula

Figure 1 Histopathology of the internal-opening in cryptoglandular anal fistula (hematoxylin and eosin). A-D: Low-power views of internal-opening tissue; E-H: Higher-power images of the boxed regions. Red dashed contours delineate gland-like structures. A 1-2 cell thick layer of spindle-shaped myoepithelial cells with goblet cells surrounding an anal gland, surrounded by florid chronic inflammatory infiltrates with fibroblast proliferation and collagen deposition. These features are consistent with a glandular origin of the fistula and were reviewed by a senior pathologist.