Serum anti-enteric neuronal antibodies in patients with achalasia and their association with clinical profiles

Figure 1 Immunofluorescence staining patterns of anti-enteric neuronal antibodies. A: Indirect immunofluorescence images of anti-enteric neuronal antibody (AENA)-positive and negative controls; B: Representative indirect immunofluorescence images demonstrating AENA negative (-), moderate-positive, and strong-positive staining intensities. Immunostaining was predominantly localized to the cytoplasm. Large yellow arrows indicate submucosal ganglia, and small yellow arrows indicate myenteric neurons (scale bar = 20 μm). AENA: Anti-enteric neuronal antibody.