Tn antigen-driven downregulation of podoplanin promotes colorectal cancer invasion

Figure 5 O-glycosylation is crucial for the function of podoplanin. A: Schematic diagram of O-glycosylation sites on podoplanin (PDPN); B and C: Western blot validation of the expression of control, wild-type (WT), and O-glycosylation-mutated (mu) PDPN; D: The cell counting kit-8 proliferation assay; E-H: Transwell assay analysis of the migration and invasion capabilities of colorectal cancer cells overexpressing PDPN; I and J: Western blot results indicate that WT-PDPN-expressing cells can inhibit the epithelial-mesenchymal transition (EMT) process, while mu-PDPN-expressing cells promote the EMT process similarly to the control cells. Ala: Alanine; PDPN: Podoplanin; WT: Wild-type; mu: O-glycosylation-mutated; ZO-1: Zonula occludens-1.