Matrix metalloproteinase 3 facilitates colorectal cancer progression by enhancing cell proliferation and invasion

Figure 2 Matrix metalloproteinase 3 regulates colorectal cancer cell proliferation, migration, invasion, and apoptosis. A: Matrix metalloproteinase 3 (MMP3) expression levels analyzed by quantitative real-time-PCR (qRT-PCR) in NCM460 and colorectal cancer (CRC) cell lines (SW480, SW620, HT-29, HCT116, LOVO); B: MMP3 expression levels analyzed by western blot in NCM460 and CRC cell lines (SW480, SW620, HT-29, HCT116, LOVO); C: Validation of MMP3 knockdown in SW480 and SW620 cells by qRT-PCR; D: Validation of MMP3 knockdown and overexpression in CRC cells by western blot; E: Wound healing assay to evaluate the impact of MMP3 on cell migration; F: Transwell assays to assess cell migration and invasion in response to MMP3 modulation; G: Colony formation assay showing the effect of MMP3 on CRC cell proliferation; H: Flow cytometric analysis of apoptosis in cells with MMP3 silencing or overexpression. MMP3: Matrix metalloproteinase 3.